Sample : 2 μL
(Blank)Tween 20 47 mg/L (H₂O)
(Model solution)
Tween 20 47 mg/L + IgG 1 g/L
(in 0.3 M NaCl aq.)
 

Sample : 2 μL
(Blank)Tween 80 57 mg/L (H₂O)
(Model solution)
Tween 80 57 mg/L + IgG 1 g/L
(in 0.3 M NaCl aq.)
 

Column       : Shodex ODP2 HP-2D (2.0 mm I.D. x 150 mm)

Eluent       : (A) 0.1 % NH3 aq. / (B) CH3CN

               High pressure linear gradient; 

               (B %) 20 %(0 to 5 min), 20 to 90 %(5 to 19 min), 90 %(19 to 24 min), 

               90 to 20 %(24 to 25 min) 

Flow rate    : 0.2 mL/min

Detector     : UV (280 nm), ESI-MS (SIM Positive) 

Column temp. : 40 ℃